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What is the purpose of flame sterilizing the inoculating loop or needle before and after using it?

What is the purpose of flame sterilizing the inoculating loop or needle before and after using it?

For each portion of the streak (3 total per plate), flame-sterilize the inoculating loop just prior to use. Also, flame-sterilize the loop just after the final streak is performed in order to prevent contamination of the bench surface and as a consideration to others in the lab who may later use the inoculating loops.

What is the importance of flaming the inoculating loop?

The flaming process prior to the inoculation is important to prevent contamination of the cultures being inoculated. The flaming process after each inoculation is important to prevent the contamination of further inoculations for which the inoculation instrument will be used.

What is the purpose of flame sterilizing the side of the petri dish during inoculation?

Use of disposable, pre-sterilized, plastic ware makes the oven less important. The flame from a gas burner effectively sterilizes small glass or metal objects, such as inoculating loops, but one must avoid “frying” the yeast by contact with objects heated in a flame.

What is the purpose of flaming the mouth of the tube by placing it at the opening of the Microincinerator?

What is the purpose of flaming the mouth of the tube by placing it at the opening of the microincinerator? The purpose of flaming the moth of the tube is to sterilize the tube. Why do you sterilize your loop before picking up the bacterial culture?

What are three reasons for aseptic technique?


  • To acquire the skill of aseptic technique in the field of Microbiology.
  • To prevent contamination of cultures and media from microbes in the environment.
  • To transfer cultures from one medium by inoculating another medium.
  • To isolate a microorganism from a mixed culture to obtain a pure culture.

What is the purpose of flaming?

The purpose of flaming is not to sterilize but to warm the opening of the bottle and create air convection currents up and away from the opening (i.e., updraft). The warm, rising air helps prevent dust particles and other contaminants from entering the bottle.

What is the danger in using direct flaming?

It may leave carbon residues on the heated object. If not heated enough, flaming may leave carbon and other chemical residues on the heated object. To reduce this risk, you may dip the object in 70% ethanol before subjecting it to an open flame. Flaming can be highly dangerous.

What are two reasons we use aseptic technique?

Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Proper aseptic technique prevents contamination of cultures from foreign bacteria inherent in the environment.

Why is the inoculating loop allowed to cool?

Sterilise the inoculating loop. The inoculating loop is sterilised by passing it at an angle through the flame of a gas burner until the entire length of the wire becomes orange from the heat. Allow the loop to cool a few seconds before contacting the inoculum to avoid killing the microorganisms.

How to prevent aerosolization in an aseptic laboratory?

Allow the disinfectant to evaporate – do not wipe dry! Use disinfectants such as alcohol (isopropanol or 70% ethanol) or phenolic compounds (o-phenylphenol) . To prevent aerosolization, or the production of a fine mist containing bacterial cells, and spread of microbial contaminants, avoid dispensing disinfectant from a squeeze bottle.

What are the keywords for aseptic laboratory techniques?

Keywords: Basic Protocols, Issue 63, Microbiology, Aseptic technique, sterile field, serological pipette, micropipettors, Pipetman, cell culture, contamination Downloadvideo file. (29M, mp4) Protocol 1. Prepare a Sterile Workspace

Why are aseptic techniques important when culturing cells?

The goals of aseptic technique are to protect the patient from infection and to prevent the spread of pathogens. Why aseptic techniques are important when culturing cells? Aseptic technique prevents contamination of cell culture and reagents from microorganisms in the environment.

How are volume transfers done in an aseptic laboratory?

Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors Erin R. Sanders1 Erin R. Sanders 1Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles Find articles by Erin R. Sanders Author informationCopyright and License informationDisclaimer