What should you always remember when handling a microscope slide?

What should you always remember when handling a microscope slide?

Your microscope slide should be prepared with a coverslip over the sample to protect the objective lenses if they touch the slide.

• Do not touch the glass part of the lenses with your fingers.
• Always keep your microscope covered when not in use.
• Always carry a microscope with both hands.

What are we supposed to do with the coarse focus?

Focus (coarse), The coarse focus knob is used to bring the specimen into approximate or near focus. Focus (fine), Use the fine focus knob to sharpen the focus quality of the image after it has been brought into focus with the coarse focus knob.

Why should you use the coarse adjustment knob first?

So if you have just loaded a new specimen and slide you need to make sure you have the lowest power objective engaged and the stage at its lowest position and use the coarse adjustment knob first to quickly get the specimen in focus.

What happens if you try to use the coarse adjustment when the 10x lens is in place Why?

What happens if you try to use the coarse adjustment when the 10X lens is in place? The focus of the specimen would be unclear with a high objective power and the stage lifted high due to the coarse adjustment. “You only use the coarse focus knob when the 4X scanning objective is in place.”

What are three things you should do when putting the microscope away?

Putting the Microscope Away

1. Turn the microscope light off.
2. Unplug the microscope.
3. Clean all materials you used with the microscope and put them away.
4. Verify that the microscope is on the lowest powered objective.

Which objective lens should always be used first?

3. When focusing on a slide, ALWAYS start with either the 4X or 10X objective. Once you have the object in focus, then switch to the next higher power objective. Re-focus on the image and then switch to the next highest power.

Why should I avoid using the coarse focus knob?

Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens. Using the coarse focus with higher lenses may result in crashing the lens into the slide.

What happens to the microscope when you move the coarse focus?

Coarse focus Use it with the lowest power objective to get the specimen approximately in focus. After that, only use the fine focus knob, even after you change to a higher-power objective. Sometimes the coarse focus know is with the fine focus knob, as on the binocular microscope in Figure 4.1.

What happens when you turn the coarse adjustment knob towards you?

By turning the coarse adjustment knob of a microscope upwards or downwards, you are actually bringing the specimen into “focus”. The coarse adjustment knob which is located on the microscope’s arm elevates or lowers the stage to make the specimen into focus or clearer in view of the observer.

What is the purpose of the coarse adjustment knob?

COARSE ADJUSTMENT KNOB — A rapid control which allows for quick focusing by moving the objective lens or stage up and down. It is used for initial focusing.

What happens if you try to use the coarse adjustment when the 40X lens is in place?

Coarse and fine adjustment The coarse adjustment knob should only be used with the lowest power objective lens. Once it is in focus, you will only need to use the fine focus. Using the coarse focus with higher lenses may result in crashing the lens into the slide.

Why should you not use the coarse adjustment knob on high power?

What should you always remember when you use the coarse adjustment knob?

Always remember that the coarse adjustment knob should only be used with the lowest power objective lens. Once it is in focus, you will only need to use the fine focus.

Is it better to use coarse or fine adjustment?

If you only have fine, you can select any value but you have a lot of steps to take in order to go from minimum to maximum. If you have both coarse and fine adjustment, you get the pros of both. Using both coarse and fine adjustments affords the user increased control.

What’s the difference between fine and coarse adjustment on a microscope?

Knowing the difference between fine adjustment and coarse adjustment knobs on the microscope is an integral part of focusing the microscope. The coarse adjustment knob quickly gets the image in focus and the fine adjustment knobs help keep the image in focus under progressively higher magnifications.

What’s the difference between fine and coarse focus?

Most coarse and fine adjustment knobs are built with coaxial control in line with one another so you can easily switch from using the coarse focus adjustment knob to using the fine focus adjustment knob.